| 학회 |
한국고분자학회 |
| 학술대회 |
2002년 가을 (10/11 ~ 10/12, 군산대학교) |
| 권호 |
27권 2호, p.324 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The Quantitative Measurement of Attachment Forces Between Cells and Polymeric Surfaces |
| 초록 |
Biodegradable polymers have received a large amount of attention in the field of medical applications. Because they were degrade in the body by simple hydrolysis of the ester backbone to non-toxic compounds. In the tissue engineering, cell adhesion onto biodegradable polymer surfaces could be controlled according to the properties chemical composition, hydrophobicity/hydrophilicity and mechanical parameter such as roughness. The understanding of the cell adhesion to biomaterials or to components of the extracellular matrix is fundamental to various biotechnological processes, as well as to normal development and tissue maintenance. The measurement of adhesive forces of cells can play an important role in investigating the mechanism of cell adhesion. Most cases, the qualitative measurements of the cell adhesive forces have been widely adopted by counting the number of cells on any surface before and after various agitations such as washing the cell-mounted surface with buffer solution, a certain amount of laminar flow, or centrifugal force. In this study, a quantitative method is proposed in measuring adhesive forces between cells and polymeric surfaces by utilizing nonlinear inverse finite element method based on cantilever beam theory. From the amount of beam deflection that occurs in detaching the cell, the reaction forces were calculated. This quantitative measurement technique can contribute to identify quantitative characteristics of biodegradable polymer surfaces by evaluating the cell-adhesive property. Also, it can be used to investigate the effect of external factors such as biochemical treatments, biomechanical stimuli on the adhesive forces. |
| 저자 |
지경수1, 김제범2, 박기동3, 신정욱*, 이진우**
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| 소속 |
1아주대, 2*인제대, 3**연세의대 정형외과 |
| 키워드 |
biodegradable; cell adhesive force; extracellular matrix
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| E-Mail |
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