| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Proteolytically degradable hydrogels with a fluorogenic substrate for studies of cellular proteolytic activity and migration |
| 초록 |
We have developed proteolytically degradable hydrogels with covalently immobilized fluorogenic protease substrates to visualize extracellular proteolytic activity and cell migration in three dimensions. Proteolytically-activated fluorogenic substrates, a dye quenched-bovine serum albumin (DQ-BSA) or the synthesized collagenase-sensitive fluorogenic substrate (CS-FS), was covalently incorporated into proteolytically-degradable and cell adhesive PEG hydrogels via photopolymerization. The fluorogenic substrates in solution and incorporated into hydrogels exhibited significantly enhanced fluorescence after exposure to enzymes. Fibroblasts seeded within these hydrogels spread in three dimensions and extended lamellipodia. Cell migration and proteolytic activity were visualized using confocal microscopy. Proteolytic activity from each fluorogenic substrate was concentrated near cell surfaces and remained present in the tracks where cell migration had occurred. |
| 저자 |
이수홍1, 정형민2, Jennifer L West3
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| 소속 |
1차바이오텍 / Rice Univ., 2차바이오텍, 3Rice Univ. |
| 키워드 |
Proteolytic activity; cell migration; fluorogenic substrate; 3D patterning
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| E-Mail |
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