| 초록 |
Proteins have been a fascinating target of many researches for its vital functional and structural roles in human body. Surface-enhanced Raman spectroscopy (SERS) is an emerging analysis method for protein studies due to its excellent sensitivity, selectivity and simplicity. Especially label-free detection of proteins by SERS provides plentiful information on binding, activity and conformational changes with minimal efforts compared to conventional analysis. In such context, we present a carboxylic acid-functionalized SERS substrate (CFSS) for protein label-free detection. Well aligned 3D gold nanowires generated strong and reproducible local E-field, and carboxylic acid on top of the SERS substrate captured proteins by producing peptide bond with N-terminus of the target. Two important biomarkers of Alzheimer’s disease, beta-amyloid and Tau protein, were also successfully immobilized on the surface of CFSS and analyzed by Raman spectroscopy. |
