| 초록 |
Lipid nanospheres (LNS) are drug delivery carriers due to their effective solubilizing capability and toxicity reduction of poorly water-soluble drugs. In this study, amphotericin B (AmB), an anti-fungal agent having poor solubility and limited clinical usage due to its lethal toxicity, was loaded to LNS by using lipids and cholesterol. PEG-coated liposomes containing AmB were prepared by incoporating a DSPE-mPEG into lipid composition of LNS. The PEG-coated LNS containing AmB showed about 70% of AmB entrapment efficiency with lipid composition of DPPC:CHOL:DPPA:DSPE-mPEG2000 at 8:4:2:0.8 molar ratios. The PEG-coated LNS had about 100±10 nm of mean particle size. Low molecule weight heparin was coated on the surface of PEG-coated LNS and their physicochemical properties were investigated. In order to study effects of PEG or heparin on in vitro toxicity of AmB-loaded LNS, cytotoxicity against human kidney cells and hemolysis against rat red blood cells were evaluated. |
