| 초록 |
B.glumae BGR1 can degrade p-coumaric acidand produce p-hydroxybenzoate as intermediate. And, Enterobacterclorae reversibly catalyzes decarboxylation of4-hydroxybenzoate. In this study, vanillate decarboxylase from Enterobacter clorae were cloned and expressed in B.glumae to produce phenol from p-coumaric acid, which isthe one of the basic monomer of lignin biomass. Engineered B.glumae converts 10 mM of 4-hydroxybenzoate to phenol with 43.6% conversion yield. And, it uses also 10 mM p-coumaric acid to produce phenol with37.7% conversion yield. Furthermore, production of phenol was improved bymetabolic engineering of B.glumae. |
