| 초록 |
Molecular imaging has been widely employed as a potent diagnostic modality for various diseases due to its capability for the visualization of molecular/biological events in vitro and vivo. In particular, optical nanoprobes have displayed great advantages to improve diagnostic accuracy by virtues of high resolution imaging. Here, as part of this effort, we devised polymeric fluorescent nanoprobes with encapsulation of a dark-quenched fluorogenic peptide (DQFP) containing a near-infrared fluorophore and a molecular quencher, in which the peptide sequence of DQFP is cleaved upon exposure to an enzyme. The nanoprobe enables to emit enzyme-specific fluorescence signals, as a result of DQFP cleavage, at only abnormal site. The utility of the nanoprobe as a diagnostic tool was evaluated by fluorescence imaging of cellular and mouse disease models. |
