| 초록 |
Fluorogenic nanoprobes capable of monitoring biological events in vivo have been widely devised for early detection, accurate diagnosis, and image-guided treatment of diseases. Matrix metalloproteinases (MMPs) have been identified as promising targets for diseases such as cancer, osteoarthritis, and arthritis, in virtue of their roles in tumor regulation and enzymatic degradation of various bioactive molecules. Precise MMPs activity detection for accurate real-time diagnosis has been challenging due to its homologous family containing highly similar sequences. Here, we synthesized a nanoprobe consisted of MMP-susceptible peptide sequence with a near-infrared (NIR) fluorescent dye and a quencher conjugated at each ends. The probe is quenched in its original state, and the fluorescence is turned on when the peptide is cleaved by MMP in the surrounding environment. This MMP-activatable nanoprobe is expected to allow visualization of the real-time MMP activity in vivo. |
