| 초록 |
MicroRNA(miR), a key molecule of endogenous RNA interference, is promising to be utilized as a therapeutic agent. In vivo delivery of miR, however, is a major hindrance as its polyanionic nature and vulnerability to serum rendering it difficult to reach targeted lesion. To overcome this obstacle, we present a self-assembled miR delivery system consisting of cholesterol-conjugated miR(cmiR) and poly ethylene glycol grafted poly ethylene imine(PEG-g-PEI). In our study, nanosized complexation of miR with PEI, suitable to effective protection of miR and its delivery into targeted lesion in vivo, was successfully synthesized by PEG grafting. Hydrophobicity of cholesterol assisted structural solidity in complex, avoiding undesirable loss of miR. Here, we report preparation of self-assembled complex. We examined delivery efficiency and validated therapeutic efficacy of complex. In conclusion, our miR delivery system proposed considerable potential for the effective in vivo delivery of miR. |
