| 학회 |
한국고분자학회 |
| 학술대회 |
2016년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
41권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Induction of osteogenic differentiation using the BMP-2 linked hydrogel and human periodontal ligament stem cells (hPDLSCs) |
| 초록 |
Recently, bone morphogenetic proteins (BMP), especially BMP-2 and BMP-7, are mainly used for bone regeneration. However, the dosage of BMP for treatments is even higher than physiological concentration because of short half-life and instability of the BMP. In this work, we synthesized the MPEG-b-(ε-caprolactone–ran-α-chloro-ε-caprolactone) (MPEG-PCL-Cl), and then chlorides of the MPEG-PCL-Cl are substituted to azides. The BMP-2 containing alkyne group was chemically linked MPEG-PCL-Cl via click chemistry. We mixed this solution with hPDLSCs and implanted to dorsum of nude mouse by subcutaneous injection. Injected the solution immediately became a gel-state. hPDLSCs were proliferated and differentiated to osteoblastic cells. We monitored osteoblastic phenotypes and gene expression through histological methods and qRT-PCR respectively. In conclusion, We confirmed that BMP-2 linked MPEG-PCL-Cl hydrogels could increase a half life and a stability of the BMP-2. |
| 저자 |
박승훈, 박지훈, 권두연, 김다연, 이해방, 김문석
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| 소속 |
아주대 |
| 키워드 |
Bone morphogenetic protein; Click chemistry; Osteogenic differentiation; Human periodontal ligament stem cell
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| E-Mail |
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