| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
hMSC using for CaP/dopa-HA gene delivery by inducing osteogenic differentiation |
| 초록 |
Human mesenchymal stem cells (hMSC) are one of the stem cells that have multipotent properties. However, use of hMSC is still limited to clinical steps. Genetic modification of hMSC is known to provide an opportunity of controlling cell type as well as their behavior. Herein, osteogenetic major protein of cDNA with cloning methods was presented to the human body to induce bone differentiation. An organic-inorganic hybrid gene delivery system supports more efficient differentiation. Calcium phosphate (CaP) gene delivery system was designed. The dopa-modified hyaluronic acid (HA) and growing CaP nanoparticles were adsorbed to act as both a gene vector and a particle stabilizer. CaP/dopa-HA nanoparticles showed significantly higher transfection in hMSCs because of the specific HA-binding to CD44+ expressed on their cell surface. CaP/dopa-HA/BMP2-pDNA achieved the desired gene expression. This study represents successful osteogenic differentiation of hMSC. |
| 저자 |
김이슬, 이정은, 임수연, 정지훈
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| 소속 |
성균관대 |
| 키워드 |
Human mesenchymal stem cells; Calcium phosphate; gene delivery; osteogenic differentiation
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| E-Mail |
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