| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Tissue engineered spinal cord using neuronal cells seeded polymeric scaffold |
| 초록 |
Brain-derived neurotrophic factor (BDNF) appears to enhance the regrowth of ascending sensory axon across spinal cord gap in adult rats. We postulated that resected segment of the spinal cord could be regenerated in adult rat gap implanting neuronal cells and BDNF associated with polymeric porous scaffold. Fabrication of nerve guidance channeled PLGA scaffold was performed by ice-particle leaching method. Neuronal cells such as that SC and OEC isolated from female Fischer rats were suspended with BDNF. Fischer rats with the various gaps (1~5 mm) created through the spinal cord transection were implanted by the PLGA/cells/BDNF. For histological evaluation, we were stained by H&E, S-100, NF, NSE. Histological examination confirmed exhibited the regenerated tissue with bundles than appeared to do axons for rats. In addition, NF-positive areas were observed in the regenerated tissue of regeneration group. Acknowledgement: This work was supported by SCRC (SC3100) from KMOST. |
| 저자 |
박기숙1, 하현정1, 윤선중1, 이일우2, 김문석3, 이종문1, 이해방3, 강길선1
|
| 소속 |
1전북대, 2대전성모병원 신경외과, 3한국화학(연) |
| 키워드 |
CNS; BDNF; neuronal cell
|
| E-Mail |
|
