| 초록 |
Methane is a major contributor to global warming and is the main component of the natural and shale gases that are considered next-generation carbon feedstock for biochemical production due to their low prices, which are equivalent to 20% of the price of glucose. The biological conversion of methane into valuable products by methanotrophs is potential eco-friendly methane oxidizing process. Recently, the existence of a complete MEP pathway of the novel methanotroph, Methylomonas sp. DH-1 was confirmed via genomic analysis. This strain is expected to be able to produce secondary metabolites from methane. In this study, we metabolically engineered Methylomonas sp. DH-1 to produce squalene, which is used as a functional health food, an ingredient in cosmetics, and pharmaceutical materials. Firstly, transformation and vector systems were developed to manipulate the genes of Methylomonas sp.DH-1, and a high transformation efficiency was obtained by developing electroporation technology suitable it. Metabolically engineered Methylomonas sp. DH-1 have three times higher squalene productivity than wild type. |
