| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Culture of nucleus-pulposus cell in PLGA scaffold modified with SIS sponge in vitro |
| 초록 |
Small intestinal submucosa(SIS) is a source of collagenous tissue with biocompatibility. In this study, we enhanced nucleus pulposus(NP) cell growth using SIS to complement lacked bioactivity of PLGA. Prepared PLGA scaffold was mixed SIS gel and cross-linked by EDC. Characterization of scaffold were measured. Scaffold seeded NP cell was cultured for several weeks. We confirmed DNA and collagen quantity in scaffold. RT-PCR was performed on type II collagen and aggrecan. Cell viability was evaluated by MTT assay. In PLGA modified with SIS sponge cell viability was high at 6 weeks than other scaffold. Gene expression of disc cell was good at SIS sponge at 1, 2 weeks, but good result showed at PLGA/SIS sponge in process of time. Also, GAG and collagen content presented similar result to RT-PCR. We suggests that PLGA scaffold modified with SIS sponge is suitable to form NP tissue. Acknowledgment : This work was supported by KMOHW (0405-BO01-0204-0006). |
| 저자 |
김순희1, 최방실1, 윤선중1, 박기숙1, 이종문1, 김문석2, 이해방2, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
PLGA; SIS sponge; intervertebral disc; Nucleus pulposus
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| E-Mail |
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