| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Effect of SIS Scaffold on Chondrogenic Differentiation for Bone Marrow-derived Stem Cells |
| 초록 | Objective: Bone marrow-derived stem cells (BMSCs) may represent a convenient source of stem cells for cell therapy and tissue engineering application. They exhibit both multipotentiality and self-renewal capabilities. Small intestinal submucosa (SIS) had been widely used as a biomaterial without immune rejection responses. In this study, we manufactured that SIS sponge prepared by crosslinking with EDC and examined into osteogenic differentiation of BMSCs into SIS sponges. Methods: Fabricated SIS sponge was cross-linking by 100 mM EDC solution. Rat-BMSCs seeded 1x106cells in sponge and cultured in vitro for 28days. We have characterized the chondrogenic potential of rat-BMSCs in SIS sponges by histology, GAG, MTT assay, SEM and RT-PCR for chondrogenic specific phenotypes. Results: In SEM, all morphology of SIS sponges showed regular and interconnected pore structure. We know that BMSCs cultured with chondrogenic media displayed significantly higher levels of GAG content compared to untreated controls. By RT-PCR analysis, we observed type II collagen (chondrocyte specific genes) expression. This result demonstrates chondrogenic differentiation of rat-BMSCs. This result indicates that SIS sponge is useful for chondrogenesis of BMSCs. Conclusion: We confirmed that SIS sponge were highly porous with good interconnections between each pore, which can support the cell adhesion, proliferation, and differentiation. In conclusion, we confirmed that SIS sponge could apply to induce chondrogenesis of BMSCs. Acknowledgement: This research was supported by SCRC (SC3100) and KMOHW (0405-BO01 -0204-0006) Reference 1. K. Lindberg, S. F. Badylak, Burns, 27, 254 (2001) 2. H. W. Shin, S. H. Kim, J. W. Jang, M. S. Kim, S. H. Cho, H. B. Lee, and G. Khang, Polymer(Korea), 28, 194 (2004) |
| 저자 | 박기숙1, 홍금덕1, 김순희1, 하현정1, 최방실1, 윤선중1, 백미옥1, 고연경1, 김문석2, 이종문1, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | Chondrogenesis; SIS; stem cell; glycosaminoglycan |
