| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 |
10권 1호, p.404 |
| 발표분야 |
생물화공 |
| 제목 |
Proteomic Analysis of Protein Expression Profiles in Response to Environmental Perturbation in Candida magnoliae producing erythritol |
| 초록 |
Erythritol is a low caloric and non-cariogenic sweetener produced industrially by Candida magnoliae. Proteomic analysis of C. magnoliae with two dimensional electrophoresis, mass spectrometry and database searching was tried to construct a proteome map and investigate the response to osmotic stress at a protein level. High osmotic condition at 300g/L influenced physiological properties of C. magnoliae and biosynthesis of erythritol. Among protein spots dominantly present at 29~96 kDa and pI 3~10, 22 proteins were identified to be involved in glycolysis, stress response and other essential metabolisms. Six proteins showed 3-times higher or less expression level in each osmotic condition. Heat shock protein Hsp60, mitochondrial RNA polymerase Rpo41 and ribonucleoside diphosphate reductase Rnr1 were down-regulated in high osmotic conditions. Bro1 interacting with components of PKC1-MAP kinase pathway and involving in the multivesicular bodies (MVB) pathway, catabolic enzyme Eno1 and a hypothetical protein were up-regulated in the same conditions. |
| 저자 |
김효진1, 이도엽1, 이대희1, 유연우2, 서진호1
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| 소속 |
1서울대, 2아주대 |
| 키워드 |
Erythritol; Candida magnoliae; Two-dimensional electrophoresis; Electrospray ionization tandem mass spectrometry; proteome; natural sweetner
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| E-Mail |
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| 원문파일 |
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