| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Synthesis and Characterization of Anti-Flt1 Peptide Hyaluronate Conjugate for the Treatment of Corneal Neovascularization |
| 초록 |
Anti-Flt1 peptide of GNQWFI has been reported to inhibit vascular endothelial growth factor receptor 1 (VEGFR1) - mediated endothelial cell migration and tube formation. In this work, anti-Flt1 peptide hyaluronate (HA) conjugate was successfully developed for the treatment of corneal neovascularization. Using tetrabutyl ammonium salt of HA (HA-TBA), water-insoluble anti-Flt1 peptide could be conjugated with HA in dimethyl sulfoxide. The formation of anti-Flt1 peptide HA conjugate was confirmed by 1H-NMR and fluorometric analyses. The average number of grafted peptide molecules could be controlled from 3 to 30 per single HA chain. According to in vitro biological activity tests, anti-Flt1 peptide HA conjugate exhibited a significant inhibition effect on the binding of Flt1-Fc to VEGF165 coated on the well. Furthermore, in vivo biological activity of anti-Flt1 peptide HA conjugate was confirmed from the inhibitory effect on corneal neovascularization in SD rats. |
| 저자 |
오은주1, 박기태1, 최준섭2, 주천기2, 한세광1
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| 소속 |
1포항공과대, 2가톨릭대 |
| 키워드 |
Hyaluronate; Anti-Flt1 peptide; Conjugation; Drug delivery; Corneal neovascularization
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| E-Mail |
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