| 초록 |
Temperature responsive poly(N-isopropylacrylamide) [PNIPAAm] has been exploited for various biomedical applications. In this work, poly(N-isopropylacrylamide-co-dopamine methacrylamide) [P(NIPAAm-co-DMAAm)] was synthesized for facile surface modification and application to cell sheet engineering. 1H nuclear magnetic resonance (NMR), Fourier transform - infrared spectroscopy (FT-IR), and gel permeation chromatography (GPC) confirmed the successful synthesis of P(NIPAAm-co-DMAAm). The lower critical solution temperature (LCST) was measured to be ca. 29.2°C by UV-Vis spectroscopy. Atomic force microscopic (AFM) imaging clearly visualized the transient phase transition of the temperature responsive polymer bound on silicon substrate by coordination bond formation. Furthermore, the adhesive and temperature responsive P(NIPAAm-co-DMAAm) could be successfully applied to the facile preparation of NIH-3T3 fibroblast cell sheets. |
