| 초록 |
Gram-negative bacteria is a serious cause of infections, which have to be rapidly and accurately diagnosed for specific and early treatment. Because conventional molecular tests based on antibodies or oligonucleotide probes, have limitations in their non-specific binding, cross-reactivity, and difficult access due to large molecular size, rapid labeling on bacterial cell wall is difficult to achieve. In order to differentiate Gram-negative bacteria from Gram-positive bacteria, we investigate on colistin (polymyxin E) due to its molecular attaching properties on lipopolysaccharide, known as the cell wall components of Gram-negative bacteria. Herein, we synthesized colisitn-Cy3 to labeling and detecting Gram-negative bacteria rapidly and accurately. In 1 hour labeling and washing process, we successfully confirmed colistin-Cy3 and Gram-negative bacteria interaction, and quantified that this bacteria makes stronger fluorescent labeling than others with fluorescent reader, microscope. |
