| 초록 |
This study was conducted to optimize the medium composition for carotenoid production by Arthrobacter sp. through response surface methodology (RSM). Arthrobacter sp. was supplied from Polar and Alpine Microbial Collection, Korea Polar Research Institute. Using a Placket-Burman design, from which yeast extract, MgSO4 and dextrose were identified as the significant factors affecting carotenoids production. RSM studies for carotenoids production have been carried out for three parameters of yeast extract, MgSO4 and dextrose concentrations. These significant factors were optimized by experiments and RSM, as 1 g/L yeast extract, 0.0879 g/L MgSO4 and 1 g/L dextrose. The experimentally obtained concentration of carotenoid was 288 mg/L, and it became 2-fold increase on concentration before optimization. |
