| 학회 | 한국화학공학회 |
| 학술대회 | 2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 | 16권 1호, p.334 |
| 발표분야 | 분리기술 |
| 제목 | Separation of Guanine and Cytosine in Three-Zone SMB(Simulated Moving Bed) Chromatography |
| 초록 | DNA play important roles in cell metabolism. Development of modern analytical technology and tool resulted in the deciphering DNA of nucleotides and bases. Among them bases change of structure and function is the main causes of inherited diseases and human cancers. Therefore, they need to achieve separation experiment and theoretical researches. Separation investigations for DNA focus on the base pairs guanine and cytosinein nucleotide. Separation of guanine and cytosine were performed by SMB (Simulated moving bed). SMB set up by connected three C18-HPLCcolumns was used to separate guanine and cytosine. Aspen simulation was performed to determine the operating variables such as switching time, raffinate and extract flow rates. The outlet streams of SMB, raffinate and extract, which were sampled and analyzed by analytical HPLC system. The analysis indicated purity values ranges from 85% to 95% according to the changes of switching time ,raffinate and extract flow rate. |
| 저자 | 박문배, 김인호 |
| 소속 | 충남대 |
| 키워드 | guanine; cytosine; SMB (Simulated moving bed) |
| 원문파일 | 초록 보기 |
