| 초록 |
In this study, in order to raise glucan content of Laminaria japonica, reaction conditions were optimized by using the response surface methodology (RSM) based on a Central Composite Design(CCD) considering reaction temperature, time, and catalyst concentration as factors. The obtained optimized conditions were the reaction temperature of 147.14℃, reaction time of 27 min 51 sec, and sulfuric acid concentration of 0.30%. The predicted glucan content was 32.83% and actually observed glucan content was 32.67% at the optimum conditions. Compared with 6.95% of glucan content in raw material, glucan content of fractionated Laminaria japonica increased about five times. Additionally, the enzyme saccharification was conducted with fractionation of Laminaria japonica to assess the enzymatic digestibility. The enzymatic digestibility of fractionated Laminaria japonica showed 92% after 48 hr at 45FPU/g glucan.
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