| 초록 |
Enzyme-linked immunosorbent assay (ELISA) is a commonly used tool for detecting and quantifying biomarkers. In the colorimetric ELISA, target molecules are captured on the surface of microplates, and enzymes linked to the detection probe catalyzes the conversion of chromogenic substrates. Herein, we report a new type of ELISA-like colorimetric bioassay inspired by the biopigmentation process in nature. Melanin is a broad term for a group of natural pigments that produce the wide variation seen in the skin, hair, and eye color of humans. The chemical structure of melanin resembles that of polydopamine, a well-known mussel-inspired adhesive used in material-independent surface chemistry. This similarity inspired us to design an enzyme-initiated colored adhesive generation for colorimetric bioassays. Unlike the conventional ELISA, the adhesiveness allows the colored pigment to be localized on the surface without diffusion making highly concentrated signal detected by the naked-eye. |
