초록 |
Biodiesel converted from lipid of microalgae is one of prospective renewable energy sources. However, microalgal biodiesel is still not cost-competitive because of the high production cost. Especially, the drying process in commonly used lipid extraction requires high energy input. For an energy-efficient extraction of biomass from microalgae, therefore, it is essential to extract the intracellular lipid directly from wet microalgae without drying the microalgal biomass to reduce the production cost. In our previous work, a novel cell disruption process was devised using a functional membrane coated with a cationic polymer. The proposed mechanism of cell disruption involves the perturbation of the local electrostatic equilibrium of the amphiphilic microalgal cell membrane caused by the direct contact with the tertiary-amine cations. This work combines the cationic polymer with shear stress using a custom-built shear stress reactor to improve the efficiency of cell rupture. |