| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 |
12권 1호, p.891 |
| 발표분야 |
재료 |
| 제목 |
The optical characterization of surface-bound Cu/Zn superoxide dismutase (SOD1) by surface plasmon resonance |
| 초록 |
The optical characterization of surface-bound Cu/Zn superoxide dismutase (SOD1) by surface plasmon resonance (SPR) measurements is described. Both time- and angle-resolved SPR curves were recorded before and after the immobilization of Cu/Zn superoxide dismutase (SOD1). SPR signals of the urea-driven denaturation and renaturation of the tethered SOD1 were generated that, unlike the upright shift in SPR curves of a bare Au thin film, the exposure to a urea solution causes denaturation of SOD1, which shifts the minimum in the SPR curve to a larger angle without any change in reflectivity at the resonant angles (ƟSPR) for different urea concentrations. The differential reflectivity at ((Rmin/Ro)) increases sigmoidally as a function of urea concentration and becomes saturated at concentrations above 4 M. Assuming a two-state model for the denaturation of SOD1, the Gibbs free energy change for the denaturation of SOD1 on the Au surface is estimated to be 1.8 0.7 kcal/mol. |
| 저자 |
강태욱, 홍수린, 김영훈, 최인희, 김윤정, 성정준, 이정상, 한지숙, 이종협
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| 소속 |
서울대 |
| 키워드 |
Protein; in vitro immobilization; surface plasmon resonance
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| E-Mail |
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| 원문파일 |
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