화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO))
권호 25권 2호
발표분야 포스터-정밀화학
제목 Design and Effect of the Degree of Cross-linking on PEG-based resins for High-purity SPPS via Microfluidics
초록 Poly(ethylene glycol) (PEG) has been prominently used as the basis of solid supports due to its hydrophilic properties. However, limitation regarding low purity of peptide synthesis still exists. To overcome the drawback, we designed polymer supports generated from microfluidic channels. The resins were prepared by dissolving various monomers and their appropriate crosslinker at varying ratios. The physicochemical properties of each resin were compared with that of the conventionally used TentaGel S NH2. Such characteristics were evaluated by assessing the: 1) swelling properties, 2) cell-adhesive behavior of RGD tripeptide-bound resins and 3) purity of a complicated Jung-Redemann decapeptide. Among these resins, PEG-based resins with a higher degree of cross-linker (PEGA:PEGDA of 3:5 ratio) presented favorable results. Hence, we anticipate that such polymer support to be used as a cost-efficient alternative for conventional resins as well as in the application of on-bead bio-assays.
저자 박지연, 신동식
소속 숙명여자대
키워드 poly(ethylene glycol); microfluidics; cross-linking; solid phase peptide synthesis
E-Mail dshin@sm.ac.kr