| 초록 |
Polydiacetylene (PDA) has been widely investigated as a sensor due to unique colorimetric and fluorescent changes by external stimuli, that is, from blue to red and from non-fluorescent to red-fluorescent. However, the quantum yield of PDA alone is very low. In order to overcome this disadvantage, we employed fluorescence resonance energy transfer (FRET). In this study, we prepared a mixed vesicle by inserting BODIPY fluorescent dye (BO558) into 10,12-Pentacosadiynoic acid (PCDA) vesicles. We report that mixed vesicle sensors can selectively detect α-cyclodextrin (α-CD) over γ-cyclodextrin (γ-CD). The fluorescence intensity of polymerized PCDA/BO558 vesicles was significantly decreased. The intensity was increased upon reaction with α-CD, while it was not almost changed upon reaction with γ-CD. Such fluorescent increases are believed to result from reduced energy transfer due to a conformational change of the conjugated backbone of PCDA by the inclusion complexation of α-CD. When compared to colorimetric response of the PCDA vesicles, the fluorescence changes of the PCDA/BO558 vesicles using FRET were more valuable in view of detection performance. |
