| 초록 |
The endothelium is a multifunctional tissue that actively participates in the maintenance of cardiovascular homeostasis. Small intestinal submucosa (SIS) is an acellular biomaterial usually derived from the jejunum of pigs. We fabricated hybrid polymeric films containing PLGA and various concentration of SIS to confirm whether the SIS affect the human aortic endothelial cells (HAECs) behavior and find suitable concentration of SIS in the hybrid film for HAECs culture. The preservation of cell phenotype on the SIS/PLGA films was confirmed by the presence of cell-specific markers, including eNOS, PECAM-1, KLF2 and VE-cadherin for HAECs at both protein and transcription levels using immunocytochemistry and RT-PCR, respectively. The results indicate a favorable interaction between vascular cells and SIS/PLGA films. These data confirm the potential of this novel hybrid films in vascular tissue engineering. This research was supported by WCU (R31-20029) and SCRC (SC4110). |
