| 학회 | 한국화학공학회 |
| 학술대회 | 2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 | 22권 1호, p.414 |
| 발표분야 | 생물화공 |
| 제목 | Gene cloning and characterization of cold-adaptive amylase from Antarctic Arthrobacter sp. |
| 초록 | The gene encoding an α-amylase from Arthrobacter sp. PAMC 27388, isolated from King George Island, Antarctica, was cloned into pET-28a(+) vector and heterologously expressed in Escherichia coli BL21 (DE3). Recombinant α-amylase protein was purified by Ni2+-NTA affinity chromatography. The optimum condition for enzyme activity was at 30℃ and pH 3.0. The α-amylase activity was stimulated by FeCl2, NaCl, KCl, β-mercaptoethanol and phenylmethylsulfonyl fluoride (PMSF), but inhibited by CoCl2, ammonium persulfate and urea. Thin layer chromatography (TLC) analysis showed that the cold-active α-amylase hydrolyzed starch, maltotetraose and maltotriose, and produced maltose as major end product. Key words : Arthrobacter, psychrophlic, cold-active α-amylase, cloning, starch |
| 저자 | 김수미, 최종일 |
| 소속 | 전남대 |
| 키워드 | 효소/단백질공학 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
