| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.459 |
| 발표분야 | 생물화공 |
| 제목 | High level production of poly(3-hydroxybutyrate) with high molecular weight by fed-batch culture of recombinant Escherichia coli |
| 초록 | Poly(3-hydroxybutyrate)[P(3HB)] is synthesized in numerous bacteria [1]. However, P(3HB) has poor properties, which are not suitable for commercial application [2]. Recently, P(3HB) having ultra-high-molecular-weight (UHMW) around 20 MDa was obtained by recombinant Escherichia coli harboring P(3HB) biosynthesis genes and was reported to have improved mechanical properties compared with typical P(3HB) [3]. In this study, we developed the fermentation strategy for the high level production of UHMW P(3HB). Recombinant Escherichia coli harboring the Alcaligenes latus P(3HB) biosynthesis genes was examined for the production of UHMW P(3HB). [This work was supported by the National Research Laboratory Program (2000-N-NL-01-C-237) of the Ministry of Science and Technology (MOST)]. References 1. Anderson, A.J., Dawes, E.A. Microbiol Rev 1990;54:450-72. 2. De Koning, G.J.M., Lemstra, P.J. Polymer 1993;34:4089-94 3. Kusaka, S., Iwata, T., Doi, Y. Int. J. Biol. Macromol 1999;25:87-94 |
| 저자 | 송애진, 박시재, 이상엽 |
| 소속 | 한국과학기술원 |
| 키워드 | poly(3-hydroxybutyrate) with high molecular weight |
| , , |
