| 초록 |
C-reactive protein (C-RP) is a pentameric protein present in a blood stream where inflammatory events occur: liver failure, leukemia and/or bacterial infection. It would be, therefore, required to measure the accurate amount of C-RP in blood since the level of C-RP indicates the progress and prognosis of diseases. The normal concentration of C-RP in blood is around 1-3 mg/L. Any inflammatory events would increase the level of C-RP up to 500 times than the one in normal condition. In our work, we demonstrated the preparation of DNA-aptamer conjugated peripheral blood mononuclear cells (Apta-PBMCs) specifically capturing human C-RP. Live PBMCs functionalized with aptamers could detect different levels of human C-RP in serum by producing immunocomplexes aggregated with anti-CRP antibodies attached to microbeads. We investigated the migratory behavior of Apta-PBMCs toward the highly concentrated C-RP sites. The immune responses of Apta-PBMCs were evaluated by measuring the profiles of TNF-alpha secretion after stimulating PBMCs with lipopolysaccharide (LPS). |
