| 초록 |
Nucleic acid has been attracted with its programmable property, Watson-Crick base pairing, for the construction of various nanomaterials containing two or three dimensional structures in bioengineering field of studies. Not only simple hybridization of DNA and RNA depending on the complementary sequences, but also enzymatic replication has been developed to amplify the nucleic acid with DNA or RNA specific polymerase to synthesize nano or semi-micron structures. As one of the powerful enzyme-driven replication, rolling circle replication (RCR) is widely used to fabricate spherical nano-sized particles which has repeated units of template circular DNA. Herein, tumor-targeted DNA and RNA nanoparticles were developed by RCR technique and coating the surfaces with tumor-specific antibodies through biotin-streptavidin interaction. These target-specific antibody-coated DNA and RNA nanoparticles can be applied as a cancer cells nanodetector and gene delivery system. |
