| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Induction of synergistic prostate cancer cell death via co-delivery of Bcl-xL shRNA and doxorubicin using aptamer conjugated smart polyplex |
| 초록 |
A synergistic combination cancer therapy was investigated upon co-delivery of shRNA against Bcl-xL and doxorubicin (DOX) using aptamer conjugated smart polyplex (ASPs). Branched polyethyleneimine grafted with polyethylene glycol (PEI-PEG) was synthesized as a vehicle for shRNA delivery and its surface was further conjugated with anti-PSMA aptamer (APT) for selective delivery of ASPs to prostate cancer cells. ASPs were finally obtained after intercalation of DOX as a form of shRNA/PEI-PEG-APT/DOX conjugates. Cell viability test and FACS analysis of pEGFP expression against PC3 and LNCaP cells proved that the synthesized ASPs were highly biocompatible and had strong cell selectivity. Consequently, IC50 values of ASPs were approximately 17-fold less than that of the simple mixture of drug and gene. These results suggest that the co-delivery of anti-cancer drug and shRNA against Bcl-xL using synthethized ASPs may widen the therapeutic window and allow selective cancer cell killing. |
| 저자 |
김은정, 정유경, 최향태, 양재문, 서진석, 허용민, 김건홍, 함승주
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| 소속 |
연세대 |
| 키워드 |
nonviral gene delivery; targeted drug delivery; shRNA; Bcl-xL; doxorubicin; aptamer
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| E-Mail |
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