| 초록 |
This study focuses on development of a multiplex pathogen detection platform based on polydiacetylene (PDA) by a novel immobilization procedure. PDA liposome based-solid sensors have held a critical drawback that the PDA liposomes are not stably immobilized to a solid substrate. Therefore, to overcome this problem, we introduced an interlinker, ethylenediamime, which acts as a cross-linker of individual PDA liposome. The quantity of ethylenediamine added was optimized to 1 mм as measured by the fluorescent signal emitted by the stably immobilized PDA liposomes, at which the fluorescence signal is 10 times higher than the resulting PDA chips made without the interlinker. The fabricated biosensor was able to perform simultaneous and quantitative detection of 6 species of pathogens. As such, we believe that the results demonstrated from this research can be exploited for development of more advanced PDA-based biosensors and diagnostics. |
