초록 |
In this study, extraction performance using mixtures of hexane and methanol were evaluated to develop a more efficient method for large-scale lipid extraction from microalgae. The combination of hexane and methanol showed the highest fatty acid methyl ester (FAME) yield for extracted lipids from Tetraselmis sp. The effects of extraction conditions including proportions of methanol to hexane, ratios of total solvent volume to dry biomass and extraction time on extraction yields were evaluated to determine cost-effective conditions providing higher lipid and FAME yields. Finally, when the amount of using large solvent, the reaction time becomes short. If the reaction time is long the equilibrium is reached even when the amount of using small solvent. The results of this study suggest that a mixture of hexane and methanol is a promising solvent for a large-scale lipid extraction from microalgae. |