| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.502 |
| 발표분야 | 생물화공 |
| 제목 | 융합 페리틴의 요소 농도에 따른 재접힘 특성에 관한 연구 |
| 초록 | Fusion ferritin(FH+FL), an iron-binding protein, was purified from recombinant E. coli by two-step sonications with urea. Unfolded ferrintin was refolded by gel filtration chromatography with various concentration of urea. 50 mM Tris-HCl(pH 8.0) buffers with 1 M to 4 M urea were used in GFC. Objective was to characterize the structure change with urea concentration. Molecular weight was determined using GF-HPLC and secondary structure was observed by CD(circular dichroism) spectroscopy. |
| 저자 | 김형원, 김인호 |
| 소속 | 충남대 |
| 키워드 | ferritin; refolding; GFC |
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