| 초록 |
Despite several researches to enhance L-tyrosine production, but control of translation-level expression and carbon flux rebalancing around phosphoenolpyruvate (PEP) nod still remain challening for achieving pathway optimization. Here, we redesigned metabolic pathway by fine-tuning gene expression levels to increase L-tyrosine production. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5’-untranslated region (5’-UTR) were introduced for each gene of interest to allow for control at both transcription and translational levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. |
