| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Precise Auxotrophic Control for Stable and Tunable Maintenance of Plasmids for Antibiotic-free Cultivation |
| 초록 |
Although plasmid-based expression systems have advantages in multi-copy expression of genes, heterogeneity of plasmid copy number (PCN) in individual cells is inevitable even with the addition of antibiotics. Here, we developed a synthetic auxotrophic system for stable and tunable maintenance of the PCN in Escherichia coli without addition of antibiotics. This auxotroph expresses infA, one of the essential genes encoding a translation initiation factor, on a plasmid instead of on the chromosome. With this system, the gene expression was stably maintained for 40 generations with minimized cell-to-cell variation under antibiotic-free conditions. Moreover, varying the expression level of infA enabled us to rationally tune the PCN by more than 5.6-fold. This antibiotic-free PCN control system significantly improved the production of itaconic acid (1.7-fold) and lycopene (2-fold) compared to the conventional system based on antibiotics. |
| 저자 |
임현규1, 강채원1, 양진아2, 서상우2, 정규열1
|
| 소속 |
1POSTECH, 2서울대 |
| 키워드 |
Synthetic auxotrophy; plasmid copy number; expression control; antibiotic-free; plasmid stability
|
| E-Mail |
|
