| 초록 |
Corynebacterium glutamicum has no endogenous metabolic activity for utilizing the D-xylose, which is second most abundant sugar-derived hemicellulose, for cell growth. Therefore, we introduced xylose isomerase pathway to xylose-negative C. glutamicum and overexpression of the pentose phosphate pathway genes and a heterologous phosphoketolase (PHK) pathway gene for xylose utilization. Overexpression of the genes encoding for tal, gnd, or xpkA improved the growth and xylose consumption rates compared to the wild-type with the XI pathway alone. The engineered strains, including overexpression of the tal, gnd, or xpkA gene alone and co-overexpression of the tal and xpkA genes, with varying xylose concentrations using a BioLector. Interestingly, xylose-utilizing C. glutamicum strains showed increased specific growth rates at higher concentrations of xylose. This study could be useful for amino acid and other value-added chemical production from xylose as the sole carbon source. |
