| 초록 |
tMesenchymal stem cells (MSCs) have a great capacity for self-renewal while still maintaining their mul-tipotency. In this study, we describe a nonviral gene delivery carrier, glycol chitosan-methyl acrylate-polyethylenimine (GMP) polymer targeted towards humanadipose-derived mesenchymal stem cells (AD-MSCs). Transfection efficiency, using luciferase and a pDNA encoding enhanced green fluorescent protein, along with cytotoxicity assays, were performed in human AD-MSCs. The results show that the transfection efficiency of the GMP polymer was similar to that of PEI25kD, and the cytotoxicity was lower. Moreover, human AD-MSCs were treated with the GMP polymer/pDNA polyplex and its cellular uptake and distribution were analyzed by flowcytometry and confocal microscopy. We found that the conjugated GMP polymer could escape from the endosome to the cytosol. The present studydemonstrates that the GMP polymer can be used as a potential targeted–delivery carrier for effectivegene delivery. |
