| 학회 | 한국화학공학회 |
| 학술대회 | 2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 | 12권 1호, p.434 |
| 발표분야 | 생물화공 |
| 제목 | Micro-dialysis of protein from urea-rich sample in microfluidic device |
| 초록 | Microfluidic technology allows the design and operation of effective and simple devices for sample preparation and cleanup. Majority of current sample preparation methods is focused on the complex and combined various steps such as solid phase extraction and membrane dialysis, and what’s more, it is still performed off-chip in many cases. As a result, we made the simple and rapid microfluidic desalting system, which were 3-phase flow micro-dialysis using the simple diffusion and 5-phase flow micro-dialysis based on the coupling simple diffusion with affinity of urea, for the effective removal of urea. The facilitative desalting system is particularly useful for removal of urea and for analysis of protein by mass spectroscopy so that it is removed urea by the mass transfer with the different of concentration and molecular size and the affinity of urea with the complex of copper(Ⅱ) ion and urea. We also evaluated the activity and MALDI-TOF-MS spectrum of red fluorescent protein (RFP) for protein analysis. This work was supported by grant No.R01-2004-000-10681-0 from the Basic Research Program of the Korea Science & Engineering Foundation. |
| 저자 | 허윤석1, 양광석1, 이은주1, 전영시1, 홍연기2, 홍원희1, 김도현1 |
| 소속 | 1한국과학기술원 생명화학공학과, 2충주대 |
| 키워드 | Microfluidic system; desalting; urea; protein |
| 원문파일 | 초록 보기 |
