| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 |
22권 1호, p.458 |
| 발표분야 |
생물화공 |
| 제목 |
Development of DNA Aptamer Specific to Salmonella typhimurium for Early Detection of Salmonellosis |
| 초록 |
Salmonella is a major foodborne pathogens and a cause of salmonellosis. Conventional detection methods are complicated and time-consuming, so that the need for rapid detection has increased. Aptamers are single-stranded oligonucleotides that can bind to their targets with high affinity and specificity. In this study, we carried out whole-cell SELEX to obtain specific ssDNA aptamers against S. typhimurium. After 10 rounds of selection, the binding affinity was confirmed by nano-drop and real-time PCR. We measured affinity of aptamers against various microbes by SPR. These aptamers can be used to early detection system for S. typhimurium. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project title: Risk Assessment Research and Development of Rapid Diagnostic Method for Biological, Chemical and Environmental Animal Disease, project No.: PJ01052301)” Rural Development Administration, Republic of Korea. |
| 저자 |
김서경1, 이상희1, 조성진1, Simranjeet Singh Sekhon1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
미생물공학 |
| E-Mail |
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| 원문파일 |
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