| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.414 |
| 발표분야 |
생물화공 |
| 제목 |
Stem Cells onPeptide NanopatternedSurface for the Detection of Toxic Effects of Olganophosphate Pesticide |
| 초록 |
We fabricated peptide nanopatterned gold electrode for increasing the affinity between the cell and electrode surface. RGD-MAP-C peptide was self-assembled on the gold electrode homogeneously via nanoporous anodic alumina oxide mask.Cells were immobilized onfabricated surface and the viability was measured by electrochemical method.Thereafter, neural stem cells were treated with two kinds of environmental toxins, and the intensities of reduction peak measured by cyclic voltammetry were decreased with the increase of concentrations of environmental toxins. These electrochemical results were confirmed by MTT assay. Our newly developed cell chip can be used as useful label-free analyzing tool for detecting drug effects or for assessing the toxicity electrochemically. Acknowledgments: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (2011-0000384) and by the Ministry of Knowledge Economy(MKE) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology. |
| 저자 |
조현열, 김태형, 최정우
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| 소속 |
서강대 |
| 키워드 |
cell chip; nanopattern; stem cell; RGD peptide
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| E-Mail |
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| 원문파일 |
초록 보기 |
