| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 |
23권 2호, p.1734 |
| 발표분야 |
생물화공 |
| 제목 |
A functional tyrosinase as a monophenol monooxygenase based on a significantly high monophenolase/diphenolase activity ratio |
| 초록 |
Tyrosinase efficiently catalyzes the ortho-hydroxylation of monophenols and the oxidation of diphenols without any additional cofactors. Although it is of significant interest for the biosynthesis of catechol derivatives, the highly fast activity as a catechol oxidase and inactivation have hampered practical utilization of tyrosinase as a monophenol monooxygenase. Here, we prepared a functional tyrosinase that exhibited a distinguished monophenolase/diphenolase activity ratio and an enhanced catalytic efficiency against L-tyrosine. This enzyme was still highly active in iced water, and its activity was well conserved below 30 °C. Taking advantage of these biocatalytic properties, in vitro DOPA modification was achieved by the enzyme, with a remarkably high yield as a monophenol monooxygenase. These results provide strong potential for use as a monophenol monooxygenase in biomedical and industrial applications. |
| 저자 |
강응수1, 도현수1, 양병선2, 차형준2, 최유성1
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| 소속 |
1충남대, 2포항공과대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
