| 초록 |
Biological functions and biocompatibility of nucleic acid made it a promising biopolymer for various biomedical applications. In this regard, a variety of nucleic acid-based nanostructures has been developed dramatically due to its sequence-specific features. Especially, nucleic acid nanoparticles (NAPs) has been spotlighted in the therapeutics area. NAPs fabricated by rolling circle replication (RCR) process had a massive amount of nucleic acids with tandem repeats of the same sequences. In this project, we improved the functional performance of NAPs by coating a calcium layer to NAPs. By directly mixing NAPs with calcium ion, the layer composed of calcium on the surface of NAPs was generated. This physical barrier could regulate the drug release rate which could prevent the initial-burst release of a NAPs-loaded drug molecule. Moreover, this calcium layer could increase cellular uptake efficiency by assisting the NAPs to bind with the cell membrane. |
