| 초록 |
MicroRNAs (miRNAs), a class of endogeneous and noncoding small RNA, play an important role in a wide range of biological processes, such as proliferation, development, organismal aging, tumorigenesis and viral infection. With the increased in the number of miRNAs shown alteration its expression during specific biological process, multiplex and quantitative analysis technology is desired to understand aging and associated pathogenesis. Despite of its importance, miRNAs are challenging molecules to amplify by PCR due to limitations in designing PCR primer for miRNAs. Cleavage-based signal amplification of RNA, recently developed method, is an exponetional isothermal amplification for the detection miRNAs with high specificity and sensitivity. Here we developed this isothermal amplification method that employ single-strand conformation polymorphism (CE-SSCP), which is suitable for multiplex analysis. We tested our new method by detecting the well-known miRNAs, like let-7, lin-4, miR-48, miR-84 and so on. |
