| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Multiplex microchip for integrating sequential operations of lipid production in microalgae |
| 초록 |
The unit cost for the production of algal biofuel needs to be reduced in order to be a substitute for fossil fuel. Here, we developed a PDMS-based multiplex microfluidic system with eight chambers and micro-pillar arrays to expedite multiple steps for lipid sample preparation from different strains. We could rapidly and efficiently perform sequential operations from cell culture to lipid extraction of eight different strains simultaneously on a single device without harvesting and purification steps by the simple injection of medium and solvent into the central inlet due to the integrated micro-pillar arrays connecting the chambers and central inlet. The lipid extraction efficiency using this system was comparable (94.5–102.6%) to the conventional Bligh-Dyer method. These results demonstrate that our multiplex microfluidic approach can provide an efficient analytical tool for the rapid analysis of strain and the determination of the optimal lipid induction condition for each strain. |
| 저자 |
최윤영, 곽호석, 심상준
|
| 소속 |
고려대 |
| 키워드 |
microalgae; biodiesel; microfluidic device; PDMS
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| E-Mail |
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