| 초록 |
RNA interference (RNAi)-based approaches hold great potential for cancer therapy. SiRNA-based therapy may allow development of a broad armamentarium of targeted drugs against genes that are difficult to target with other traditional approaches. However, one of the key challenges to the use of siRNA for therapy is the need for efficient delivery system. Therefore, we developed siRNA-incorporated chitosan nanoparticles (siRNA-CH-NP) to increase delivery efficiency of siRNA in tumor endothelial cell. The size of siRNA-CH-NP was 159 ± 10 nm and the efficiency of siRNA encapsulation was up to 80%. The siRNA-CH-NP showed therapeutic efficacy in mouse tumor models. In addition, we demonstrate PLXDC1 targeted gene silencing in tumor endothelial cell. This approach resulted in significant inhibition of tumor growth and targeted gene silencing. |
