| 학회 | 한국화학공학회 |
| 학술대회 | 2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 | 18권 1호, p.402 |
| 발표분야 | 생물화공 |
| 제목 | Development of GABA overproducing recombinant Escherichia coli by engineering of glutamate decarboxylase GABA transporter |
| 초록 | GABA, or gamma-Aminobutyric acid is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of L-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.47 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.7%. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology (2011-0022392). |
| 저자 | 고지선, Tam Dinh Le Vo, 이해룡, 홍순호, 유익근 |
| 소속 | 울산대 |
| 키워드 | GABA; recombinant Escherichia coli; glutamate decarboxylase |
| 원문파일 | 초록 보기 |
