| 초록 |
Biorefinery is leading to a new manufacturing paradigm for the industrial synthesis of chemicals from renewable biomass. However, one of the challenges is a low-yield conversion process. In this study, the native redox cofactor regeneration system in Escherichia coli was engineered to implement non-native synthetic pathway for production of butyric acid. This chemical is common substrate of 1-butanol, which is considered for a replacement for gasoline. The engineered strain JHL26, which regenerates NAD+ from NADH using butyrate as the only final electron acceptor enabled high-yield production of butyric acid from glucose (83.4% of the molar theoretical yield) with a high selectivity (butyrate/acetate ratio of 41). This study could be broadly utilized for the production of various other useful chemicals in the fields of metabolic engineering and synthetic biology. |
