| 초록 |
Oral delivery has the potential to easy administration and increase drug absorption for type 2 diabetes. To increase DNA condense between lgG Fc fragment and GLP-1 gene, nine arginine groups conjugated with C-terminus of human lgG1 Fc fragment (hlgG-Fc-9Arg) because Arginine is cationic amino acid. An antibody- complex penetrates into the cells by interacting with cell receptors, it is important to understand their biological roles and requirements for efficient targeting. In this study, we showed an efficient ratio of hlgG-Fc-9Arg/pDNA to cellular uptake and release therapeutic proteins such as Glucagon-like peptide 1(GLP-1) in FcRn receptor expressed cells in vitro. An in vivo type 2 diabetes treatment study by oral administration of hlgG1-Fc-9Arg/pDNA complexes showed absorption in GI track to balb/c male mice. The oral delivery of hlgG-Fc-9Arg/pDNA complexes to target cell delivery appears a promising approach to achieve potent treatment of type 2 diabetes. |
